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WELCOME TO CHEMICAL PATHOLOGY DEPARTMENT

HOURS OF DUTY

The laboratory operates 24 hours services on every day of the week. The laboratory is fully staffed for routine laboratory requests between 8.00am – 4.00pm (Monday- Friday). Call duty/emergency requests are attended to between 4.00pm-8.00am (Monday- Friday), Saturdays, Sundays and on public holidays. The call duty requests are strictly emergency tests


TYPES OF SPECIMEN/SAMPLES

The laboratory accepts the following types of samples- blood, urine (random and timed), stool, cerebrospinal fluid, amniotic fluid exudates and aspirates.


For the purpose of this handbook;

Samples to be collected in lithium heparin are designated LH

Samples to be collected in fluoride oxalate are designated with FB

Samples to be collected into plain bottles are designated PB

Samples to be collected in universal bottles are designated UB

Samples to be collected in containers to be provided by the patients are designated PC


TESTS AVAILABLE

The following tests are carried out in the laboratory


Renal Function Tests

  1. Electrolytes –Sodium, Potassium, Bicarbonate (Lithium Heparin)- LH
  2. Urea LH
  3. Creatinine LH
  4. Uric acid LH
  5. Clearance tests- Creatinine clearance, Urea clearance. LH & PC
  6. Urinary sodium, urinary potassium. PC

Liver Function Tests Profile

  1. Total bilirubin LH
  2. Conjugated bilirubin LH
  3. Total protein LH
  4. Albumin LH
  5. Globulin LH
  6. Alkaline phosphatase LH
  7. Alanine transferase SGPT LH
    Aspartate transferase SGOT LH
  8. Lactate dehydrogenase (LDH) LH
  9. ɣ- glutamyl transferase LH

Cardiac Function Tests/strong>

  1. Fasting blood glucose Flouride oxalate-FB
  2. Oral glucose tolerance test (OGTT) FB
  3. Glycated haemoglobin LH
  4. Total cholesterol LH
  5. High density lipoprotein (HDL) LH
  6. Low density lipoprotein (LDL) LH
  7. Triglyceride LH

REPRODUCTIVE HORMONE PROFILE

  1. Follicle stimulating hormone (FSH) PB
  2. Leutinizing hormone (LH) PB
  3. Oestrogen/ Oestradiol PB
  4. Progesterone PB
  5. Testosterone PB
  6. β-HCG PB

THYROID FUNCTION TESTS PROFILE

  1. Thyroid stimulating hormone (TSH) PB
  2. Triiodothyronine (T3) PB
  3. Tetraiodothyroxine (T4) PB
  4. Free T3 PB
  5. Free T4 PB
  6. Thyroglobulin PB

BONE ASSESSMENT TESTS/ PROFILE

  1. Calcitonin PB
  2. Calcium LH
  3. Inorganic phosphate LH

ADRENAL AND PARATHYROID TEST PROFILE

  1. Parathyroid hormone (PTH) PB
  2. Cortisol PB

SPECIAL TESTS

  • Acid phosphatase LH
  • Protein electrophoresis PB
  • Cerebrospinal fluid (CSF) test profile
    1. CSF glucose FB
    2. CSF protein LH
  • Urinary protein UB
  • Occult blood test UB
  • Neat pregnancy test UB
  • Dilution pregnancy test UB
  • Prostate specific antigen (PSA) PB
  • Insulin PB
  • Microalbumin LH
  • Lithium
  • Magnesium LH
  • Alpha-feto protein PB
  • Bence Jones protein UB
  • Total iron binding capacity (TIBC) PB
  • Ca-125 PB
  • C-reactive protein PB

URINALYSIS UB

Glucose, protein, bilirubin, ketone bodies, urobilinogen, porphobilinogen, bile salts, pH, specific gravity, Urine osmolality UB


CALL DUTY TESTS

These are tests that are attended to during call duty hours, weekends and public holidays

  1. Glucose
  2. Sodium
  3. Potassium
  4. Bicarbonate
  5. Direct bilirubin
  6. Total bilirubin
  7. CSF glucose
  8. CSF protein
  9. Urea

PATIENTS PREPARATION

1. Proper patients’ preparation is highly essential for quality results, thus all concerned with laboratory requests should kindly adhere to adequate patients’ preparation


Such tests include the following: OGTT, 24hr urine collection, occult blood test, pregnancy test, fertility hormone profile, cortisol,

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Instructions for 24 hours urine collection.

The starting time of collection (not later than 7.00am) should be noted. The first morning urine should be discarded. All urine voided throughout the 24 hour period should be collected into the special container (keg) containing preservative (earlier added from the Laboratory). At the end of 24 hours period (e.g 7.00pm), the next urine sample voided should be added even if the patient does not feel the urge to urinate immediately. The accompanying blood sample should be collected not later than 1 hour prior to the end of the urine collection.


Instructions for fasting and two hours postprandial blood glucose collection.

A normal evening meal should be taken the night before the test is scheduled which should not be later than 8.00pm. After this no water or food should be taken until the end of the test the following morning. Fasting blood sample should be collected not later than 8.00 am on the day of the investigation and the sample should be clearly labelled 'FASTING' with time of collection written on the sample bottle. Then patient should eat his/her normal breakfast. The exact time of finishing the breakfast should be noted on a paper and patient should not eat or drink anything till the two hours postprandial sample is collected. The postprandial sample should be collected exactly 2hours after the breakfast and be labelled ‘POSTPRANDIAL’. The two samples should be brought to the laboratory immediately after collection. If urine sample is requested, it should be collected before the breakfast is taken.


Instructions for collection of stool for occult blood test.

The patient should avoid blood meal, blood containing meal, blood tonic, multivitamins supplements minimum of three days before stool sample collection. In addition, female patient should not be menstruating


Instructions for pregnancy test.

Early morning urine should be collected in clean sample container.


SAMPLE COLLECTION

Proper sample collection is an integral pre-requisite to quality laboratory results.


Out-patients samples

Samples from out-patients will be collected between 8.00-1.00pm


In-patients samples

Samples from in-patients will be collected 24 hour round but requests that come in after 1.00pm (Monday-Friday) will be treated the following day except when such samples are for emergency requests.


Tests that need laboratory preparations

The laboratory should be pre-informed of any special request (e.g lithium) or when any special procedures involving laboratory collaboration are to be carried out e.g organ transplant.


Sample transportation to the Laboratory

The integrity of sample reaching the laboratory is essential for quality results. The following steps are to be adhered to for proper sample transportation.

  1. Sample should be transported with a hold in upright position to avoid spillage
  2. Samples must be transported at a low temperature in a container
  3. Samples should be transported to the laboratory not more than 1hour after collection.
  4. Infectious samples should be adequately labelled with “biohazard tape”.
  5. from the above, it is clear that only trained personnel can effectively transport samples to the laboratory.

Therefore, the laboratory will henceforth accept in-patients samples transported by only trained personnels.


TURN AROUND TIME OF TESTS PANELS

Test Panels Turnaround time
Sodium,potassium,bicarbonate,urea 8 hours
Glucose,bilirubin,urinalysis
Lipid profile,creatinine,uric acid 24 hrs (except weekends)
Liver enzymes 48 hrs
Calcium,phosphate,glycated Hb 72 hrs


Investigations with low requests

PSA 1 Week
Hormonal assays 2 Weeks

CALL DUTY INVESTIGATIONS

Blood Glucose 4 hours
Sodium, Potassium, Bicarbonate
Urea
C.S.F. protein

SAMPLE REJECTION CRITERIA

Standard practice and demand for quality results, necessitates that unsuitable samples be rejected from the reception point.


Following are the criteria for sample rejection:

  1. unlabeled or mislabeled sample
  2. duplicate samples
  3. leaking samples blood/sample stained request form(s)
  4. inappropriate sample source (urine collected instead of blood)
  5. sample collected at a wrong time
  6. sample collected in a wrong sample bottle
  7. delayed sample transport time
  8. inadequate sample volume collected
  9. request form inappropriately or not fully completed
  10. unsuitable sample (e.g haemolysed, clotted, samples)

NOTE:

Rejected sample(s) will be withheld in the laboratory and documented. The request form(s) will be returned with reason for rejection written on it for a repeat sample with new request form.


CONTACT NUMBERS/PERSONS

The following numbers can be reached in case of any enquiry:
Director (MLS): +234 8152092781,
Desk number:

COMPONENT HEALTH FACILITIES

  • IHU, ILE-IFE
  • WGH, ILESA
  • DENTAL UNIT, OAU
  • UCHC, ELEYELE, ILE-IFE
  • MULTIPURPOSE, ILESA
  • RCHC, IMESI-ILE

SCHOOLS

  • Community Health Officers’ Training Programme.
  • Darkroom Technicians and Assistants Course.
  • School of Health Information Management
  • Schools of Nursing, Midwifery and Peri-operative Nursing.
  • Residency Training Programme

CONTACT US

P M B 5538, ILESA ROAD, ILE-IFE.